{
  "lockedAtUTC": "2026-10-13T15:58:23.132599+00:00",
  "models": {
    "M4_v5": {
      "path": "backend/models/m4-decay-response-v5/M4_v5.joblib",
      "sha256": "b565e5c5e1166764d44467442364e3f5011520f8f0438ae71eecb18142494266"
    },
    "ExtraTrees": {
      "path": "backend/models/m4-decay-response-v5/ExtraTrees.joblib",
      "sha256": "9b3b0d9ca94e41580b6927dfb03efaa3221ac5ba65c154f5e3ea3a3867a82acf"
    },
    "ridge": {
      "path": "backend/models/m4-decay-response-v5/ridge.joblib",
      "sha256": "1832ca7c0077b5c31291a7f5b7b36b428363ef6f466077dad169308a203dd9d4"
    }
  },
  "predictionSha256": "306c094bf21e022595be2780add8477111e3d7987f7f7df5fae61f751bb570e4",
  "geneMapSha256": "d7ad7fe5bbc3cc81a20f6053be92ef29d46c8bb9504a616d72cc5873bc0edbbc",
  "trainingSourceSha256": "08527f163c57d28fc248a78eb7f9c262dafed686300727a4257df2e90d2ff68b",
  "study": "GSE289489 / EZbakR HEK293 SMG1 inhibition",
  "endpoint": "log2(kdeg SMG1i / kdeg DMSO), gene-level GRAND-SLAM estimates processed by study authors with EZbakR",
  "sourceFile": "https://ndownloader.figshare.com/files/53217122",
  "sourceChoice": "Published Figure2.R explicitly uses EZbakRFit_GRANDSLAM_11j.rds for gene-level analysis. Chosen before numerical outcomes; avoids isoform-to-gene averaging. It is 21.7 MB versus the alternative 821 MB object, beyond available workspace capacity. Do not select an estimator by benchmark score.",
  "primaryMetric": "MAE; secondary Spearman and R2",
  "cohort": "All finite gene-level differences in the correct SMG1i/DMSO contrast with an exact stable Ensembl gene ID (version stripped) or unique exact reference gene-name mapping and frozen input predictions. Exclude ambiguous or duplicate mappings entirely. No significance, direction, magnitude or winning-subgroup filtering.",
  "targetTransform": "Published difference is natural-log kdeg contrast; divide by ln(2). Confirm reference and treatment from object metadata before scoring; stop if ambiguous.",
  "confidence": "2000 paired gene bootstrap resamples; baseline MAE minus M4 MAE; 97.5% intervals for two primary comparisons; descriptive conditional on published estimates.",
  "freshStudy": true,
  "directUPF1Holdout": false,
  "frozenBeforeOutcomeAccess": true,
  "limitations": [
    "Human SMG1 pathway transfer, not direct UPF1 depletion or therapeutic efficacy.",
    "Previously published aggregate findings were inspected for qualification; numerical gene outcomes were not opened before prediction lock.",
    "Public independent source study, no third-party blinded custody or raw-read duplicate audit.",
    "Existing input bank derives from development response availability and limits coverage; many genes may be previously seen.",
    "Uses published GRAND-SLAM/EZbakR gene estimates, not raw-read re-quantification; approximation and annotation differences remain."
  ]
}